Thermo Fisher Scientific A-Raf Polyclonal Antibody
A-Raf 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 Immunocytochemistry에 적합합니다. Human 및 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 고순도(>95%)로 연구용으로 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein of human ARAF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803921 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity, broadly expressed in nearly all cell lines, and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus.
Unregulated kinase activity of v-Raf is associated with transformation and mitogenesis, while Raf-1 activity is normally suppressed by a regulatory N-terminal domain.
A-Raf, a second member of the Raf gene family, exhibits substantial homology to Raf-1 within the kinase domain but less homology elsewhere. Expression of A-Raf is highest in urogenital tissues and kidney and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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