Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), Brilliant Ultra Violet 805, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 496, 563, 737
• Brilliant Violet 421, 605
• eFluor 450, FITC
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by thymocytes, T helper cells, and monocytes. CD4 serves as a receptor for HIV, and the SK3 clone blocks HIV binding and mixed lymphocyte reactions. SK3 and RPA-T4 recognize distinct epitopes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry using human peripheral blood cells
• Recommended Use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 805 (BUV805) is a tandem dye emitting at 797 nm, optimized for cytometers with a 355 nm UV laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific interactions.

• Light Sensitivity: Protect from light to prevent photo-induced oxidation
• Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C
• Methanol Compatibility: Suitable for short-term methanol fixation (<1 hour)

Excitation: 355 nm
Emission: 797 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD4 is a co-receptor for HIV and interacts with MHC class II molecules. It is mainly expressed in T-helper lymphocytes, but also found in monocytes, macrophages, dendritic cells, and various tissues including thymus, lymph nodes, tonsils, spleen, and specific regions of the brain and gut. CD4 aids in T-cell activation and may mediate neuronal damage in immune-related diseases. Multiple alternatively spliced transcripts are known.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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