Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells, and a subset of B cells. CD5 expression by B-1 cells defines a distinct lineage of B cells. CD5 acts as a counter-receptor for CD72 and plays a role in T-B cell interaction.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes (≤ 0.25 µg/test).
A test is defined as the amount of antibody that stains cells in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

CellBlox Plus Blocking Buffer:
Updated formulation required for optimal staining with NovaFluor conjugates.
Add 5 µL per sample to antibody cocktails/master mixes before cell incubation.
For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton™ technology utilizing nucleic acid dye structures for engineered fluorescent signatures, minimizing spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD5 is a 67 kDa transmembrane glycoprotein found on mature T-lymphocytes, thymocytes, and certain B cells. It regulates T-cell proliferation and is associated with T-cell leukemias and lymphomas.
Diseases linked to CD5 dysfunction include thymus cancer and Richter’s Syndrome.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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