Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by most thymocytes, mature T cells, and a subset of B cells. CD5 expression on B-1 cells marks a distinct lineage involved in T-B cell interactions as a counter-receptor for CD72.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested with mouse splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration recommended for optimal assay performance.

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which improves staining performance.
Add 5 µL per sample to antibody cocktails or single-color controls (100 µL cell sample, 10³–10⁸ cells).

NovaFluor conjugates use Phiton™ technology, employing nucleic acid dye structures engineered for optimized fluorescent signatures with minimized spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD5 is a 67 kDa transmembrane glycoprotein found on mature T-lymphocytes, thymocytes, and certain B-cell subsets. It regulates T-cell proliferation and is expressed on 95% of thymocytes and 72% of peripheral blood lymphocytes. CD5 reactivity is observed mainly in T-cell areas of lymph nodes and is associated with T-cell leukemias, lymphomas, and activated T cells.
Diseases linked to CD5 dysfunction include thymus cancer and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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