Thermo Scientific™

GeneBLAzer™ TRα-UAS-bla HEK 293T Assay Kit

TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to자세히 알아보기

TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. TR alpha-UASbla HEK 293T 293 cells are functionally validated for Z` and EC50 concentrations of T3 Thryoid hormone. In addition, TR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

사양

대체 작용제9-cis-retinoic acid, TTNPB, CD437, AM 580

길항제AGN 193109

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Dividing Cells

검출 방법Fluorescence

일차 작용제All-trans-retinoic Acid

제품라인GeneBLAzer™

수량1 Plate

리포터 유전자BLA (Beta-Lactamase)

타겟 입력THRA, TR alpha, NR1A1

유형Detection Assay Kit

세포주HEK 293T

System TypeGeneBLAzer™

Druggable TargetNuclear Receptors

Unit SizeEach