Thermo Fisher Scientific Beta-catenin Monoclonal Antibody (UMAB14), UltraMAB
Beta-catenin 단백질을 인식하는 UMAB14 클론의 mouse monoclonal antibody. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 세포 접착 및 Wnt 신호 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| ChIP assay (ChIP) | Assay-Dependent |
| Peptide Array (Array) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB14 |
| Immunogen | Full-length human recombinant protein of CTNNB1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Beta-catenin is a key adherens junction (AJ) protein involved in cell-cell adhesion and epithelial layer formation. It interacts with E-cadherin and alpha-catenin to link cell adhesion complexes to the actin cytoskeleton.
Beta-catenin also associates with adenomatous polyposis coli (APC) protein and participates in Wnt/Wingless signaling pathways, influencing morphogenesis and cell proliferation.
Pathological roles have been identified in pilomatrixoma, medulloblastoma, colorectal, ovarian, and other solid tumors. In the nucleus, beta-catenin co-activates Lef/Tcf transcription factors, promoting genes such as cyclin D and c-myc that drive proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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