Thermo Fisher Scientific SNAP alpha Polyclonal Antibody
Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합한 rabbit polyclonal antibody. Human, mouse, rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태. 4°C 단기 보관, -20°C 장기 보관 권장. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 78–295 of alpha SNAP (Uniprot ID: P54920) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11157675 |
Product Specific Information
- Recommended positive controls: HepG2, mouse brain, rat brain
- Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (89%), Pig (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SNAPs (soluble NSF attachment proteins) act with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF) to mediate vesicle fusion with target membranes. This process is crucial in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion, and transcytotic vesicle-to-plasma membrane transport.
Vesicle docking occurs when vesicle SNAREs bind to target SNAREs. α-SNAP (or β-SNAP in brain) binds to this docking complex, facilitating NSF binding and forming a 20S fusion particle. ATP hydrolysis by NSF initiates the membrane fusion process.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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