Thermo Fisher Scientific CD304 (Neuropilin-1) Monoclonal Antibody (3DS304M), eFluor 450, eBioscience

Thermo Fisher Scientific CD304 (Neuropilin-1) Monoclonal Antibody (3DS304M), eFluor 450

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 2 publications

Product Specifications

Additional Formats

• APC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The monoclonal antibody 3DS304M recognizes mouse CD304 (Neuropilin-1), a type 1 transmembrane glycoprotein expressed on multiple cell types including Treg, NKT cells, dendritic cells, stem cells, neurons, endothelial cells, and neoplastic cells.
Neuropilin-1 acts as a co-receptor for type 3 semaphorins and participates in angiogenesis through VEGF receptor complexes. In mice, Neuropilin-1 is expressed on thymus-derived natural Treg but not on peripherally induced Treg. Transient expression may occur on recently activated non-regulatory T cells.

This antibody recognizes formaldehyde-fixed and methanol-treated epitopes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry of mouse splenocytes at ≤0.125 µg/test
  A test is defined as staining in 100 µL final volume (10⁵–10⁸ cells/test). Optimize titration for best assay performance.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Verify instrument compatibility.
Laser: Violet Laser (405 nm)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Neuropilin-1 is a 923 amino acid membrane-bound coreceptor belonging to the neuropilin family, containing two CUB domains, two F5/8 type C domains, and a MAM domain. It forms heterodimers with NRP2 and binds plexin (PLXNB1) to form semaphorin receptors.
Primarily expressed in the nervous system, Neuropilin-1 is involved in:

• Cardiovascular development and angiogenesis
• Axon guidance and neuronal circuit formation
• Cell survival and migration
• Organogenesis outside the nervous system

It acts as a receptor for semaphorins and VEGF, regulating VEGF-induced angiogenesis. Neuropilin-1 may be associated with advanced prostate carcinoma and breast cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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