Thermo Fisher Scientific PFKL Polyclonal Antibody
PFKL 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody로, WB, IHC, ICC/IF에 사용 가능. Human 및 Mouse 반응성 확인됨. 고순도 항원 친화 크로마토그래피 정제 제품으로 안정적 저장 가능.
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Thermo Fisher Scientific PFKL Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 428–674 of PFKL (Uniprot ID: P17858) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.23 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_11155219 |
Product Specific Information
- Recommended positive controls: A431, H1299, HeLa, HepG2
- Predicted reactivity: Mouse (95%), Rat (95%), Chicken (88%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Phosphofructokinase (PFK) is a major regulatory enzyme that catalyzes sugar metabolism, pivotal in energy production for normal cell function. In humans, three loci control PFK expression: M (muscle), L (liver), and P (platelet) type subunits, variably expressed across tissues.
PFK forms tetramers from combinations of M and L subunits, resulting in five possible isozymes. It is activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.
Deficiency in PFK can cause episodic illness, characterized by dark urine after strenuous exercise. Elevated red cell PFK levels are observed in individuals with Down syndrome.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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