Thermo Fisher Scientific CD1a/HTA1 Recombinant Mouse Monoclonal Antibody (rC1A, 711)
인간 CD1a 단백질을 인식하는 재조합 마우스 단일클론 항체입니다. IHC(P) 및 펩타이드 어레이에 사용 가능하며, 고순도의 Protein A/G 정제로 제조되었습니다. PBS(pH 7.4) 용액 형태로 제공되며 방부제 무첨가, -20°C 이하 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rC1A, 711 |
| Immunogen | Recombinant full-length human CD1a protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD1a is a non-polymorphic MHC Class I-related cell surface glycoprotein expressed in association with beta-2 microglobulin. It is found on cortical thymocytes, Langerhans cells, and interdigitating cells, as well as in certain T-cell malignancies and histiocytosis X.
CD1a is structurally related to MHC proteins and forms heterodimers with beta-2-microglobulin. CD1 family proteins mediate the presentation of lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes (CD1a–e) organized in a cluster on chromosome 1, differing in localization and ligand specificity. CD1a localizes to the plasma membrane and early endocytic vesicles. Alternatively spliced variants have been observed, though their biological significance remains unclear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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