Thermo Fisher Scientific Lamin B2 Monoclonal Antibody (GT144)
Lamin B2 단백질을 인식하는 Mouse monoclonal antibody (Clone GT144). Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. Human에 반응하며, 단백질 G로 정제된 액상 형태. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT144 |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 277–600 of Lamin B2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.81 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538740 |
Product Specific Information
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, HepG2, HepG2 nuclear
- Predicted reactivity: Bovine (83%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic side of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins.
A-type lamins (lamin A, lamin C, lamin Adel 10) arise from the same gene by alternative splicing, while B-type lamins (lamin B1 and lamin B2) originate from two distinct genes.
Mutations in A-type lamins are associated with several rare genetic disorders such as Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease, and Dunnigan-type familial partial lipodystrophy.
The expression of A-type lamins is linked to cell differentiation, and their interaction with chromatin suggests a role in regulating differential gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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