Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), Biotin, eBioscience
인간 및 비인간 영장류 FOXP3 단백질 검출용 단클론 항체. Biotin 결합 형태로 흐름세포분석, 면역조직화학 등에 적합. CD4+CD25+ 조절 T세포 분석에 유용하며, 고순도 친화 크로마토그래피 정제 제품. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | - | 23 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 |
| Flow Cytometry (Flow) | 0.5 µg/test | 21 |
| Immunoprecipitation (IP) | - | 1 |
| Radioimmune Assays (RIA) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rhesus monkey |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 236A/E7 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466655 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC
- eFluor 450
- eFluor 570
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- Request custom conjugation available
Product Specific Information
The 236A/E7 antibody reacts with human Foxp3 protein, also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 (49–55 kDa) is a member of the forkhead/winged-helix family of transcriptional regulators and is the gene defective in scurfy (sf) mice. High expression of Foxp3 mRNA is observed in CD4+CD25+ regulatory T cells (Treg), and ectopic expression in CD4+CD25− cells imparts a Treg phenotype.
Intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) show staining of the CD4+CD25^bright population. The epitope recognized by 236A/E7 differs from that of PCH101 (Product #72-5776-40). This antibody also recognizes rhesus macaque, sooty mangabey, and cynomolgus macaque.
Applications Reported:
Intracellular staining followed by flow cytometric analysis and immunohistochemistry (paraffin and frozen sections).
Applications Tested:
Tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00). Refer to BestProtocols® for “Staining Intracellular Antigens for Flow Cytometry” (Protocol B). Recommended use ≤0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is expressed highly in CD25+CD4+ regulatory T cells and minimally in CD4+/CD25− cells, absent in CD4−/CD8+ T cells. FOXP3 acts as a master regulatory gene and specific marker of Treg cells. Mutations cause the scurfy phenotype in mice and IPEX syndrome in humans (immune dysfunction, polyendocrinopathy, enteropathy, X-linked). FOXP3 expression is p53-regulated in human breast and colon cancer cells.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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