Thermo Fisher Scientific Calcium Green-1 dextran, Potassium Salt, 10,000 MW, Anionic

Invitrogen™ Calcium Green™-1 dextran, Potassium Salt, 10,000 MW, Anionic

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. They are widely used in calcium signaling studies, including:

• Measuring intracellular Ca²⁺ concentration
• Monitoring Ca²⁺ influx and release
• Multiphoton excitation imaging of Ca²⁺ in living tissues

Cells can be loaded with the cell-impermeant salt forms of these dextran-conjugated indicators using patch pipette, microinjection, or the Influx pinocyte-loading reagent. The resulting fluorescence signal is measured using fluorescence microscopy.

Dextran-conjugated calcium indicators show significantly reduced leakage and compartmentalization compared to AM ester forms.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Specifications

Spectral Characteristics of Molecular Probes™ Calcium Indicators

These probes are excited by visible light, reducing the risk of photodamage. They are efficiently excited by laser-based instruments such as confocal microscopes, and their emissions occur in spectral regions with low cellular autofluorescence and scattering background.

More Choices for Fluorescent Calcium Indicators

Thermo Fisher Scientific offers a wide range of Molecular Probes™ calcium indicators for various experimental needs, including:

• Dextran versions for reduced leakage and compartmentalization
• BAPTA conjugates for detecting high-amplitude calcium transients

For more information, refer to the Fluorescent Ca²⁺ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

For UV-excitable Ca²⁺ indicators, protein-based Ca²⁺ indicators, and indicators for other metal ions (Mg²⁺, Zn²⁺), see Indicators for Ca²⁺, Mg²⁺, Zn²⁺ and Other Metal Ions—Chapter 19.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.