Thermo Fisher Scientific Placental Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody (PD00-19)
Recombinant rabbit monoclonal antibody (clone PD00-19) recognizing human placental alkaline phosphatase (PLAP). Validated for WB and IHC-P. High specificity and reproducibility. Supplied as liquid, 1 mg/mL, in PBS with BSA and glycerol. For research us...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:8,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PD00-19 |
| Immunogen | Recombinant fragment within Human Placental Alkaline Phosphatase (PLAP) aa 150–300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4 °C short term; for long-term storage, store at −20 °C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931117 |
Target Information
Placental Alkaline Phosphatase (PLAP) plays an important role in the regulation of inflammatory processes. There are four related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney types. PLAP is a membrane-bound isoenzyme (Regan and Nagao type) expressed in the placenta during the third trimester. It is a useful marker for identifying testicular germ cell tumors. PLAP-positive somatic cell tumors typically express epithelial membrane antigen (EMA). Although PLAP is proposed to contribute to matrix mineralization, mice lacking this enzyme show normal skeletal development. Mutations affecting PLAP are linked to hypophosphatasia, a disorder characterized by hypercalcemia and skeletal abnormalities, with variability depending on mutation type.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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