Thermo Fisher Scientific Rabbit anti-Human IgG (H+L) Secondary Antibody, AP
Rabbit 유래 Human IgG(H+L) 검출용 AP 결합 2차 항체로 Western blot, IHC, ELISA에 적합. 고순도 IgG fraction 정제, 높은 특이성과 감도 제공. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific Rabbit anti-Human IgG (H+L) Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| ELISA | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgG whole molecule |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | 0.05M Tris HCl (pH 8.0), 1mM MgCl₂, 1µM ZnCl₂, 50% glycerol, 10mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10981826 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
PA1-28837 detects Human IgG from human samples.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting, or purification of their specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems such as HRP, AP, and fluorescence. They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats Available
- Biotin
- FITC
- HRP
- Texas Red
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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