Thermo Fisher Scientific CD8 Monoclonal Antibody (3B5), Pacific Orange

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 20 publications 20 publications

Miscellaneous PubMed (Misc)

-

View 14 publications 14 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Human CD8

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

View additional formats

• Unconjugated
• Alexa Fluor 405
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-Alexa Fluor 750
• FITC
• Pacific Blue
• PE
• PE-Alexa Fluor 610
• PE-Cyanine5.5
• PE-Cyanine7
• PE-Texas Red
• PerCP
• Qdot 605
• Qdot 655
• Qdot 705
• Qdot 800
• TRI-COLOR
• Request custom conjugation

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372066

Product Specific Information

Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

This antibody recognizes the alpha chain alone as well as the alpha/beta heterodimer.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

Analyte Specific Reagent