Thermo Fisher Scientific DGKD Polyclonal Antibody
Thermo Fisher Scientific의 DGKD Polyclonal Antibody는 인간 DGKD 단백질을 인식하는 토끼 유래 다클론 항체입니다. Western blot과 면역세포염색(ICC/IF)에 적합하며, 친화 크로마토그래피로 정제되었습니다. PBS/glycerol buffer에 보관되며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DGKD (Accession Q16760), corresponding to amino acid residues S46–V96 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903013 |
Product Specific Information
This antibody detects endogenous levels of total DGKD.
Target Information
Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. Both DAG and phosphatidic acid act as lipid second messengers in signaling cascades.
Different DGK isoforms exhibit distinct expression patterns and biological roles:
- DGK-alpha: Regulates cell activation and exosome secretion, influencing cell death.
- DGK-beta: Highly expressed in brain regions such as the caudate putamen and olfactory tubercle.
- DGK-gamma: Expressed in various tissues, with highest levels in the retina.
- DGK-delta: Abundant in skeletal muscle.
- DGK-epsilon: Specific for arachidonyl-containing diacylglycerol, mainly in testis.
- DGK-theta: Found in cerebellum and hippocampus.
- DGK-iota: Predominantly expressed in brain and retina, with multiple transcript variants.
- DGK-eta: Closely related to DGK-delta.
- DGK-zeta: Abundant in brain and muscle.
DGKs contain structural motifs that play regulatory roles, forming the basis for classification into five subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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