Thermo Scientific™

Pierce™ Avidin Agarose

Thermo Scientific Pierce Avidin Agarose can be used in a variety of applications for the affinity purification of biotinylated macromolecules.Features자세히 알아보기

Thermo Scientific Pierce Avidin Agarose can be used in a variety of applications for the affinity purification of biotinylated macromolecules.

Features of Avidin Agarose:

• Strong , nearly irreversible biotin:avidin interaction
• Binding biotinylated anti-transferrinfrom serum (1)
• Binding biotinyl peptides and elution with a SDS/Urea solution (2)
• Hybridization of biotinylated RNA to its complementary DNA and binding to immobilized avidin, with subsequent elution of the single-stranded DNA (3)
• Purification of double-stranded DNA (4)
• Approximate Binding Capacity >20 μg biotin/mL resin

In one variation, an antibody that has an affinity for a particular antigen is labeled with biotin. Cells containing the antigen are lysed, then incubated with the biotinylated antibody to form a typical antigen/antibody complex. To isolate the antigen, the crude mixture is passed through an immobilized avidin or streptavidin column, which will bind the complex. After appropriate washes, the antigen can then be eluted from the column with a pH 2.8 elution buffer. The biotinylated antibody is retained by the column.

Related Products
Pierce™ Monomeric Avidin Agarose

사양

용량(미터법)20 μg Biotin Binding per mL Resin

제품라인Pierce™

수량5 mL

타겟Biotin-Tagged Molecule

유형Agarose

정지상Avidin

형식Bottle

컬럼 유형Affinity, Agarose Resin

Unit SizeEach