
Thermo Fisher Scientific Mouse anti-Human IgG3 (Hinge region) Secondary Antibody
Mouse monoclonal secondary antibody specific to human IgG3 hinge region. Suitable for WB, IHC(F), Flow Cytometry, and ELISA. Unconjugated form, 1 mg/mL concentration. Provides high specificity and sensitivity for detection of human immunoglobulins.
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Thermo Fisher Scientific Mouse anti-Human IgG3 (Hinge region) Secondary Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Frozen) (IHC(F)) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | HP6050 |
| Immunogen | Purified human IgG3 myeloma protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C or -20°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2538209 |
| Target | IgG3 |
| Antibody Form | Whole Antibody |
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specific targets. Secondary antibodies offer increased versatility, enabling use with various detection systems (e.g., HRP, AP, fluorescence). They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species. They can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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