Thermo Fisher Scientific CD80 Monoclonal Antibody (OTI10D8), TrueMAB
CD80 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot 및 Immunocytochemistry에 사용 가능. Human CD80 full-length 단백질을 면역원으로 제작되었으며, 동결건조 형태로 제공. 연구용으로 T 세포 활성화 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10D8 |
| Immunogen | Full length human recombinant protein of human CD80 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for the T cell costimulatory molecule CD28 and the inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation.
CD80 is rapidly induced on activated B cells and expressed in EBV-transformed B cell lines, Burkitt's lymphoma cells, follicular B lymphoma cells, T cells, and monocytes.
The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes through interaction with CD28 and CTLA4.
CD80 dysfunction has been associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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