Thermo Fisher Scientific CD80 Monoclonal Antibody (OTI7H7), TrueMAB
CD80 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western Blot 및 Immunocytochemistry에 적합합니다. 인간 시료에 반응하며, 무보존제 동결건조 형태로 제공됩니다. 재구성 후 약 1 mg/mL 농도로 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7H7 |
| Immunogen | Full length human recombinant protein of human CD80 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation.
CD80 expression is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes.
B7-1 provides regulatory signals for T lymphocytes via CD28 and CTLA-4 interactions.
CD80 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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