Thermo Fisher Scientific Phospho-TPL2 (Ser400) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of MAP3K8/COT only when phosphorylated at Ser400.

Target Information

MAP3K8 (COT) is a serine/threonine kinase that activates both MAP and JNK kinase pathways and plays a role in the cell cycle.
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK).
MAPKKK/MEKK phosphorylates and activates MAPK kinase/MEK, which in turn activates MAPK.
These kinases are highly conserved across species, including yeast, Drosophila, and mammals.
MEKK8 activates NF-kappa-B1 by stimulating proteasome-mediated proteolysis of NF-kappa-B1/p105.
This cytoplasmic protein is expressed in various normal tissues and tumor-derived cell lines.
The 58 kDa form is activated during the S and G2/M phases of the cell cycle.
The longer form is phosphorylated mainly on Ser residues, while the shorter form on both Ser and Thr residues.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

제품 이미지

(이미지 제거 후 하단에 배치됨)