Thermo Fisher Scientific eIF4E2 Polyclonal Antibody
Rabbit polyclonal antibody against human eIF4E2, validated for WB, IHC, and ICC/IF. Recognizes 7-methylguanosine-containing mRNA cap and functions as a translation initiation repressor. Supplied as liquid, 1 mg/mL, for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein of human EIF4E2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C for short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2719892 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
eIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap during the early step of translation initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349). It acts as a repressor of translation initiation (PubMed:22751931). Unlike EIF4E, it does not bind eIF4G (EIF4G1, EIF4G2, or EIF4G3), suggesting it competes with EIF4E and blocks eIF4F assembly at the cap. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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