Thermo Fisher Scientific CD59 Monoclonal Antibody (MEM-43), Biotin
인간 CD59(Protectin)에 특이적인 Mouse monoclonal antibody (Clone MEM-43)로, Biotin으로 결합됨. IHC, Flow Cytometry, IP 등에 사용 가능. 1 mg/mL 농도의 액상 시약으로, PBS(pH 7.4) 내 15mM sodium azide 포함. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Flow Cytometry (Flow) | 1–2 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-43 |
| Immunogen | Thymocytes and T lymphocytes |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1073159 |
Available Formats:
- Unconjugated (MA1-19133)
- Alexa Fluor 647 (MA5-18148)
- APC (A15705)
- FITC (MA1-19576)
- PE (MA1-19639)
- Custom conjugation available upon request
Product Specific Information
This antibody reacts with a well-defined epitope (W40, R-53) on CD59 (Protectin), an 18–20 kDa GPI-anchored glycoprotein expressed on all hematopoietic cells and widely present in all tissues.
It does not compete with MEM-43/5 and exhibits blocking activity.
Target Information
CD59 (Protectin) is a small (18–20 kDa) GPI-anchored inhibitor of the membrane attack complex (MAC).
It prevents complement-mediated cell lysis by binding complement C8 and/or C9 during MAC assembly, inhibiting cytolytic pore formation.
CD59 also acts as a low-affinity ligand for human CD2, mediating T cell costimulation and lymphocyte signaling.
Mutations in CD59 can cause CD59 deficiency, associated with hemolytic anemia, thrombosis, and cerebral infarction.
Multiple alternatively spliced transcript variants exist.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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