Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104), Brilliant Violet 786, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed on all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes
  • Recommended ≤ 1.0 µg/test (in 100 µL final volume)
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)
  • Titrate for optimal performance

Brilliant Violet™ 786 (BV786) emits at 786 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant UV™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  for up to 3 days at 4°C in dark with minimal brightness loss.
• Not compatible with methanol-based fixation.*

Excitation: 407 nm
Emission: 786 nm
Laser: Violet laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells (~10% of lymphocyte surface proteins).
It regulates Src-family kinases, is essential for lymphocyte development and antigen signaling, and exists as multiple isoforms via alternative splicing.
These isoforms differ in extracellular domains and expression depending on cell type and physiological state.
CD45.2 also modulates integrin-mediated signaling and participates in apoptosis-related DNA fragmentation.
CD45RA is an isoform with restricted expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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