Norgen FFPE RNA Purification Kit Dx

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FFPE 조직 샘플에서 총 RNA 및 microRNA를 신속히 추출하는 CE-IVD 인증 진단용 키트. 페놀 없이 고순도 RNA 확보, DNase 포함으로 DNA 제거 가능. 모든 크기의 RNA 정제에 적합하며 RT-qPCR, NGS 등 다양한 후속 분석에 활용 가능.

브랜드: Norgen
카테고리: Other Organics
판매단위: pk

#Norgen #Reagents #RNA Reagents #R

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Norgen FFPE RNA Purification Kit Dx판매 단위 pk ·

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743,000원VAT 포함 817,300원

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Norgen · Norgen FFPE RNA Purification Kit Dx

Norgen FFPE RNA Purification Kit Dx

SKU: Dx25300
For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples for in vitro diagnostic use.

Features and Benefits

CE-IVD marked in accordance with EU Directive 98/79/EC
Ideal for use in in vitro diagnostic workflows
Extract total RNA (including microRNA) from FFPE samples
No phenol extraction step
Includes DNase for optional on-column DNA removal
Isolated RNA is of the highest quality and integrity
Isolate a diversity of RNA species
Purified RNA is suitable for downstream applications including Small RNA Sequencing
Purification based on spin column chromatography using Norgen’s proprietary resin matrix

Product Description

Norgen’s FFPE RNA Purification Kit Dx provides a rapid method for isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. The kit enables partial reversal of formalin-induced modifications, yielding high-quality RNA suitable for diagnostic and research applications. It purifies all sizes of RNA from large mRNA and rRNA down to microRNA (miRNA) and siRNA.

Supporting Data

Figure 1. High Quality and Yield of Total RNA
Norgen’s kit isolates FFPE RNA with higher yield and quality than competitors.
Total RNA was extracted from hamster FFPE kidney sections and analyzed via Agilent BioAnalyzer.
Norgen samples exhibited higher RIN values.

이미지 OCR 데이터 (Figure 1)

항목	내용
샘플명	Sample 3
비교 대상 1	Norgen (RIN = 6.5)
비교 대상 2	Competitor A (RIN = 2.9)
세로축 단위	FU
가로축 단위	nt
세로축 범위	0 ~ 25
가로축 범위	25 ~ 4000

Figure 2. Higher Yield of FFPE RNA
Norgen’s kit consistently yields more RNA compared to competitors.
Eighteen replicates were performed for each product; Norgen showed the highest mean RNA yield.

이미지 OCR 데이터 (Figure 2)

Sample	RNA Yield (approx.)
Competitor A	약 2.5 µg
Norgen	약 3.5 µg
Competitor Q	약 3.0 µg

Figure 3. High Yield of Diverse RNA Species
Norgen’s kit effectively recovers all sizes of RNA, performing well in qPCR detection of both large (beta-actin) and small (miR-21) RNAs.

이미지 OCR 데이터 (Figure 3)

구분	대상	축 정보
A	beta-actin	PCR Base Line Subtracted dRFU (0~44, 100~1000)
B	miR-21	PCR Base Line Subtracted dRFU (0~44, 100~1000)

Figure 4. Excellent Performance in RT-qPCR
Norgen’s kit yields RNA of superior quality for downstream applications such as RT-qPCR.

이미지 OCR 데이터 (Figure 4)

그래프 제목	X축 항목	Y축 항목
miR-21	Competitor A / Norgen / Competitor Q	Delta Ct
5S rRNA	Competitor A / Norgen / Competitor Q	Delta Ct
beta-Actin	Competitor A / Norgen / Competitor Q	Delta Ct

Kit Specifications

항목	내용
Maximum Column Binding Capacity	Up to 50 µg RNA
Maximum Loading Volume Per Spin Column	650 µL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	1–4 hours*
Maximum Starting Material	5 slices (<20 µm thick) or 25 mg unsectioned block
Average Yield	~2–3 µg Total RNA per 1 mg of fresh FFPE hamster kidney

* Time varies by Proteinase K incubation and crosslink reversal.

Storage Conditions and Product Stability

All solutions should be stored tightly sealed at room temperature.
DNase I and Proteinase K should be stored at -20°C upon arrival.
Stable for at least 2 years unopened.

Kit Components

Component	Cat. 25300 (50 preps)
Digestion Buffer	20 mL
Binding Solution	20 mL
Enzyme Incubation Buffer	6 mL
Wash Solution	22 mL
Elution Solution	6 mL
Proteinase K	12 mg
DNase I	200 μL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1