Thermo Fisher Scientific SIAH1/SIAH2 Monoclonal Antibody (8G7H12)
인간 SIAH1/SIAH2 및 드로소필라 SINA 단백질을 특이적으로 검출하는 마우스 단클론 항체입니다. Western blot, IP, RIP 등 다양한 응용에 적합하며, 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunoprecipitation (IP) | 1:10–1:500 | - |
| RNA Immunoprecipitation (RIP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly, Human, Pig, Rat, Zebrafish |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8G7H12 |
| Immunogen | Synthetic peptide made to a C-terminal portion of the Drosophila SINA protein (within residues 280–331) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Tissue culture supernatant |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2717254 |
Product Specific Information
This antibody is specific for human SIAH1 and SIAH2, as well as Drosophila SINA in proliferating cells and stem cells. It does not detect Drosophila SINAH or normal, nondividing cells.
Target Information
SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1 and SIAH-2, which share a conserved C-terminal sequence and differ in their N-terminal regions.
SIAH-1 is a 32 kDa protein containing an N-terminal RING-finger domain required for proteolysis and a cysteine-rich C-terminal domain that regulates oligomerization and binding to target proteins.
As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates its degradation via the ubiquitin-proteasome pathway. It also binds Bag-1, a Bcl-2 related protein, inhibiting cell growth.
Most SIAH-1 is localized in the nucleus, with a small portion in the cytoplasm, suggesting interaction with nuclear matrix proteins and DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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