Thermo Fisher Scientific SIAH1/SIAH2 Monoclonal Antibody (8G7H12)

Applications

Product Specifications

Product Specific Information

This antibody is specific for human SIAH1 and SIAH2, as well as Drosophila SINA in proliferating cells and stem cells. It does not detect Drosophila SINAH or normal, nondividing cells.

Target Information

SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1 and SIAH-2, which share a conserved C-terminal sequence and differ in their N-terminal regions.
SIAH-1 is a 32 kDa protein containing an N-terminal RING-finger domain required for proteolysis and a cysteine-rich C-terminal domain that regulates oligomerization and binding to target proteins.
As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates its degradation via the ubiquitin-proteasome pathway. It also binds Bag-1, a Bcl-2 related protein, inhibiting cell growth.
Most SIAH-1 is localized in the nucleus, with a small portion in the cytoplasm, suggesting interaction with nuclear matrix proteins and DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.