Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), PerCP-Cyanine5.5, eBioscience
Mouse CD25(IL-2Rα) 검출용 Rat IgG1 모노클로날 항체로 PerCP-Cyanine5.5 형광 표지. Flow cytometry에 최적화되어 있으며 0.25 µg/test 권장. 4°C 암소 보관, 0.09% sodium azide 포함. 다양한 형광 및 비표지 포맷 제공.
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Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), PerCP-Cyanine5.5, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 121 publications |
| Functional Assay | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_914323 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 563, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue 585, 610-70S, 660-120S, 760
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα).
CD25 is expressed by early progenitors of T and B lineage as well as activated mature T and B lymphocytes.
By itself, CD25 binds IL-2 only with low affinity, but associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high-affinity IL-2 receptor.
Binding of IL-2 to both high and low affinity receptors is inhibited by the PC61.5 antibody.
CD25 plays a role in lymphocyte differentiation and activation/proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.25 µg/test
- Excitation: 488 nm
- Emission: 695 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD25 (IL2RA) encodes the interleukin-2 receptor alpha chain, a cytokine receptor involved in T and B lymphocyte proliferation.
The receptor complex consists of IL2RA, IL2RB, and IL2RG forming a high-affinity IL-2 receptor.
IL-2 acts as a growth and differentiation factor, regulating immune cell activation and apoptosis.
Mutations in IL2RA are associated with immune dysfunctions such as interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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