Thermo Fisher Scientific TRAF5 Polyclonal Antibody
TRAF5 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. HeLa 및 Jurkat 세포에서 양성 대조로 권장되며, 인간 반응성을 가지며 마우스, 개, 소에 예측 반응. 항원 친화 크로마토그래피로 정제된 고품질 연구용 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–186 of Human TRAF5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.76 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545181 |
Product Specific Information
- Recommended positive controls: HeLa, Jurkat
- Predicted reactivity: Mouse (84%), Dog (91%), Bovine (90%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins such as TNF receptor associated factors (TRAFs). TRAFs are cytoplasmic adapter proteins that mediate signal transduction from TNF-receptor superfamily members (e.g., RANK, CD30, CD40) and the interleukin-1 receptor.
The carboxy-terminal region of TRAFs is essential for self-association and receptor interaction following ligand-induced oligomerization. Six TRAFs (TRAF1–TRAF6) have been identified.
TRAF5 (558 amino acids) is involved in NF-κB and c-Jun N-terminal kinase activation by TNF receptor superfamily members including CD27, CD30, CD40, and lymphotoxin-beta receptor. Disruption of the TRAF5 gene causes defects in CD40-CD27 mediated lymphocyte activation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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