Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 3 publications 3 publications

ELISA (ELISA)

0.125-1.0 µg/mL

View 58 publications 58 publications

Neutralization (Neu)

Assay-dependent

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

XMG1.2

Immunogen

Recombinant mouse IFN gamma

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• PE-Alexa Fluor 610
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223608

Product Specific Information

MM700B targets IFN gamma in ELISA, IHC-F, Neutralization and WB applications and shows reactivity with mouse and human samples.

The MM700B immunogen is recombinant mouse IFN gamma.

MM700B detects IFN gamma which has a predicted molecular weight of approximately 16 kDa.

The MM700B mouse GMCSF antibody (biotinylated conjugate of clone XMG1.2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM700 (same clone). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody MM700B (clone XMG1.2) and antibody MM700 (same clone) have successfully been used in combination with recombinant mouse IFNg protein SMIFNg in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.