Thermo Fisher Scientific NEK4 Monoclonal Antibody (2E9)
Thermo Fisher Scientific의 NEK4 단클론 항체(2E9)는 인간 NEK4 단백질을 특이적으로 인식하는 Mouse IgG2a 항체로, Western blot 및 ELISA에 적합합니다. 친화 크로마토그래피로 정제되었으며, PBS(pH 7.4) 용액 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E9 |
| Immunogen | NEK4 (AAH63044, 680–781 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
DVPVANPVSE FKLHRKYRDT LILHGKVAEE AEEIHFKELP SAIMPGSEKI RRLVEVLRTD VIRGLGVQLL EQVYDLLEEE DEFDREVSVS LTVSRCLCYR IF
Target Information
NEK4 is a serine/threonine kinase involved in signal transduction and cellular communication. It acts predominantly upon threonine residues and is expressed in most primary carcinomas.
Protein kinases are enzymes that transfer a phosphate group from ATP to an acceptor amino acid in a substrate protein, mediating signal transduction in eukaryotic cells. They regulate processes such as metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.
The protein kinase family includes more than 500 gene products and is divided into 8 major groups based on catalytic domain sequences. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of kinases related to MAPK cascade families (Ste7/MAP2K, Ste11/MAP3K, Ste20/MAP4K). MAP kinase cascades, including MAPK and upstream kinases (MAPKKs), are well characterized in the yeast pheromone response pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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