Thermo Fisher Scientific LEF1/TCF1 alpha (Transcription Factor) Recombinant Rabbit Monoclonal Antibody (LEF1, 341R)
Recombinant rabbit monoclonal antibody targeting human LEF1 transcription factor. Validated for IHC (paraffin). Provides high specificity and sensitivity. Suitable for research use in Wnt signaling and cancer-related studies.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | LEF1, 341R |
| Immunogen | Recombinant fragment (around aa100–200) of human LEF1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
LEF1 encodes a transcription factor belonging to a family of proteins sharing homology with high mobility group protein-1. It binds to a site in the T-cell receptor-alpha enhancer to confer maximal enhancer activity. LEF1 is involved in the Wnt signaling pathway and may function in hair cell differentiation and follicle morphogenesis. Mutations have been found in sebaceous tumors and other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송 정보
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