Thermo Fisher Scientific TPMT Monoclonal Antibody (OTI2A2), TrueMAB
TPMT 단백질을 인식하는 Mouse monoclonal 항체로 Western blot, IHC, ICC/IF에 적합. Human 시료 반응성, Lyophilized 형태로 제공되며 재구성 후 1 mg/mL 농도 사용 가능. Thiopurine 대사 관련 연구용으로 최적화.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A2 |
| Immunogen | Full length human recombinant protein of human TPMT produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TPMT catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Defects in TPMT cause thiopurine S-methyltransferase deficiency, affecting metabolism of immunosuppressive or cytotoxic thiopurine drugs used in conditions like leukemia, autoimmune diseases, and organ transplantation.
Patients with reduced TPMT activity are at risk of toxicity from standard thiopurine doses, with genetic variation at the TPMT locus being a major determinant of individual response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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