Thermo Fisher Scientific CD14 Monoclonal Antibody (LPSR, 2408)
CD14 단백질을 인식하는 Mouse IgG1 단일클론 항체로, 인간 시료에 반응합니다. Western blot, IHC, Peptide array 등 다양한 응용에 사용 가능하며 비결합형 액상 형태로 제공됩니다. 4°C 보관, Protein A/G 정제, 연구용으로 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LPSR, 2408 |
| Immunogen | Recombinant fragment of human CD14 protein (around aa 25–148) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature (RT).
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at RT for 20 minutes.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and is found both in membrane-bound and soluble forms. CD14 binds lipopolysaccharide via lipopolysaccharide-binding protein (LBP) and plays a key role in immune response modulation. Soluble CD14 (sCD14) can distinguish structural variations in lipopolysaccharides and contributes to neutralization of serum lipopolysaccharides. Additionally, CD14 binds apoptotic cells and influences allergic, inflammatory, and infectious processes. Alternative splicing results in multiple transcript variants encoding the same isoform.
Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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