Thermo Fisher Scientific NUP35 Polyclonal Antibody
Rabbit polyclonal antibody targeting human NUP35 (nucleoporin 35kDa). Suitable for WB, IHC, IHC-P, and IP applications. Provides high specificity and predicted cross-reactivity with rat. Recommended shelf life 1 year; store at 4°C.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 276 and 326 of human nucleoporin 35kDa (NUP35, UniProt ID: Q8NFH5-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) recommended for FFPE tissue sections
- Predicted to react with rat based on 100% sequence identity
Target Information
Nucleoporin Nup35 (Mitotic phosphoprotein 44) is a nuclear protein located at the nuclear pore complex, tightly associated with the nuclear membrane and lamina. It belongs to the Nup53 family and contains an MPPN (mitotic phosphoprotein N-terminal) domain. NUP35 has scattered phenylalanine-glycine repeats but lacks large FG domains typical of other nucleoporins.
Although its precise function remains unclear, NUP35 is thought to serve as a structural component of the nuclear pore complex (NPC) and as a docking partner for nuclear transport factors. It may also be involved in the association of MAD1, a transcriptional repressor, with the NPC.
NUP35 is widely expressed across various tissues.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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