Thermo Fisher Scientific LentiArray Human Whole Genome CRISPR Library

Invitrogen™ LentiArray™ Human Whole Genome CRISPR Library

The award-winning Invitrogen™ LentiArray™ Human Whole Genome Library targets 18,453 human genes, with up to 4 gRNAs per gene target (pooled in a single well), for a total of 73,812 gRNAs.
Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target with an average titer of 1×10⁸ TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks (Cat. No. A32185).

Key Features

• Ideal for whole-genome surveys to identify novel biological and disease-related targets
• Gene targets selected using up-to-date databases: NCBI RefSeq, Gene Ontology Consortium (GO), and HUGO Gene Nomenclature Committee (HGNC)
• Built with Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm for high editing efficiency and specificity
• Up to four high-quality gRNAs per gene for robust knockout efficiency across diverse cell types

Built to Bring You Success

LentiArray CRISPR libraries use Thermo Fisher Scientific’s advanced gRNA design algorithm, incorporating the latest research and in-house expertise.
Each gRNA is optimized for:

• Maximal editing efficiency
• High specificity
• Knockout of all known isoforms of the target gene

Design and Execute Experiments Without Limitations

LentiArray CRISPR libraries employ a two-vector design, expressing Cas9 nuclease and gRNA on separate lentiviral constructs.
This enables flexible experimental timing and control over genome editing delivery.

Cas9 Lentivirus Construct Features:

• Human codon-optimized Cas9 nuclease
• Blasticidin-resistance gene under the EF-1α (EFS) promoter
• Option to co-infect cells with both Cas9 and gRNA lentiviruses or use Cas9 lentivirus alone to establish stable Cas9-expressing cell lines

Additional Advantages:

• gRNA lentiviruses contain no fluorescent markers, allowing use of any reporter wavelength and multiplexed experiments
• Puromycin resistance gene included for enrichment of edited cell populations and stronger phenotypes

Optimize Your Assay Design with LentiArray Controls

High-quality controls are essential for successful screening.
The LentiArray CRISPR library product line includes a suite of controls to:

• Accelerate assay development
• Provide confidence in hit identification and validation

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