Thermo Fisher Scientific ATRX/RAD54 (Alpha Thalassemia Mental Retardation) Recombinant Rabbit Monoclonal Antibody (ATRX, 2900R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed):

• Incubate antibody for 30 minutes at room temperature.
• For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.

Target Information

ATRX encodes a protein containing an ATPase/helicase domain and belongs to the SWI/SNF family of chromatin remodeling proteins.
Mutations in this gene are associated with X-linked mental retardation (XLMR) syndrome, often accompanied by alpha-thalassemia (ATRX) syndrome.
These mutations can alter DNA methylation patterns, linking chromatin remodeling, methylation, and gene expression during development.
ATRX undergoes cell cycle–dependent phosphorylation, regulating its nuclear matrix and chromatin association, suggesting roles in gene regulation during interphase and chromosomal segregation in mitosis.
Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.