Thermo Fisher Scientific PLCG2 Monoclonal Antibody (2D9E8)
PLCG2 단백질을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, Flow Cytometry, ELISA에 사용 가능. 정제된 재조합 인간 PLCG2(aa 826–985)를 면역원으로 사용. Protein G로 정제된 액상 형태이며, 1 mg/mL 농도. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D9E8 |
| Immunogen | Purified recombinant fragment of human PLCG2 (aa: 826–985) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898512 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in receptor-mediated signal transduction through the generation of the second messengers inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.
There are many mammalian PLC isozymes, including PLCβ1, PLCβ2, PLCβ3, PLCβ4, PLCγ1, PLCγ2, PLCδ1, PLCδ2, and PLCε.
PLCδ exists as four isoforms. PLCδ1, a calcium signal amplifier, is activated by an atypical GTP-binding protein and acts as an effector in oxytocin receptor and α1B-adrenoreceptor signaling. It is highly expressed in brain, heart, lung, and testis. PLCδ is abnormally accumulated in brains with Alzheimer’s disease, suggesting a role in AD pathology.
PLCδ2 is markedly expressed in type II intestinal metaplasia and adenocarcinoma. When expressed in type I intestinal metaplasia, it is generally benign but may evolve toward neoplastic transformation, suggesting PLCδ2 as a potential marker of gastric malignant transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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