Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), APC, eBioscience
인간 CD45 전 아이소폼을 인식하는 2D1 클론 단일클론 항체. APC 형광단백질 결합으로 유세포분석에 최적화. 633–647 nm 여기, 660 nm 방출. 친화 크로마토그래피 정제, 4°C 암소 보관. 연구용으로만 사용.
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 34 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D1 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10714837 |
Additional Available Formats
- Alexa Fluor 700
- APC-eFluor 780
- Brilliant Violet 711
- eFluor 450
- FITC
- NovaFluor Blue / Red / Violet / Yellow series
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation
Product Specific Information
Description:
The 2D1 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase activity and plays an important role in lymphocyte activation.
Applications Reported:
Validated for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test. A test is defined as the amount of antibody that stains cells in a 100 µL final volume, with 10⁵–10⁸ cells/test.
Fluorescence Properties:
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. Absent in non-hematopoietic tissues, CD45 is critical for lymphocyte development and antigen signaling. It regulates Src-family kinases, JAK kinase activity, and integrin-mediated signaling, influencing immune responses, apoptosis, and cytokine signaling. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.