Thermo Fisher Scientific MTDH Monoclonal Antibody (OTI2A6), TrueMAB
MTDH 단백질을 인식하는 Mouse monoclonal antibody로 Western blot에 적합. 인간 시료에 반응하며, 동결건조 형태로 제공. PBS buffer(8% trehalose) 보존, -20°C 보관 권장. 연구용으로 breast cancer 관련 연구에 활용 가능.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 70–582 of human MTDH produced in SF9 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Metadherin (MTDH, LYRIC) is a metastasis adhesion protein localized with tight junction proteins ZO-1 and occludin in polarized epithelial cells. It is recruited during tight junction maturation rather than acting as a structural component.
MTDH is overexpressed in breast cancer tissues and xenografts, with low expression in normal breast tissue. It binds to lung vasculature, a common site of breast cancer metastasis. Blocking this interaction or inhibiting MTDH expression has been reported to reduce metastatic potential.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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