
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 650
DyLight 650 형광으로 표지된 Goat anti-Mouse IgG (H+L) 2차 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 마우스 IgG 중 Heavy 및 Light chain에 반응하며 높은 특이성과 감도를 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 650
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse IgG, whole molecule |
| Conjugate | DyLight™ 650 |
| Excitation/Emission Max | 651/673 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_10942301 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #84545 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
This antibody reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. It may cross-react with immunoglobulins from other species.
Store the product protected from light at 4°C until opened. To extend shelf-life, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
DyLight 650 Amax = 654 nm; Emax = 673 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of their specified targets.
Secondary antibodies offer versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
They are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Agarose
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- AP
- Biotin
- DyLight 350
- DyLight 405
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 633
- DyLight 680
- DyLight 800 4X PEG
- FITC
- HRP
- PE
- Rhodamine
- TRITC
- WesternDot 625
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