Thermo Fisher Scientific LIG3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 254-460 of human DNA Ligase III/LIG3 (NP_0392692) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNA Ligase III,uniProtId:P49916-1,ncbiNodeId:9606,antigenRange:254-460,antigenLength:1009,antigenImageFileName:PA5-109910_DNA_Ligase_III_P49916-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109910_DNA_Ligase_III_P49916-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.62 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855321

Product Specific Information

Immunogen sequence: CDPRHKDCLL REFRKLCAMV ADNPSYNTKT QIIQDFLRKG SAGDGFHGDV YLTVKLLLPG VIKTVYNLND KQIVKLFSRI FNCNPDDMAR DLEQGDVSET IRVFFEQSKS FPPAAKSLLT IQEVDEFLLR LSKLTKEDEQ QQALQDIASR CTANDLKCII RLIKHDLKMN SGAKHVLDAL DPNAYEAFKA SRNLQDVVER VLHNAQE

Target Information

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100 kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97 kDa (beta form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.