Thermo Fisher Scientific Human LXR-a, Ligand Binding Domain, GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Ligand Binding Assay (LBA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

207-447

Tag

GST-tag

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

proprietary buffer

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

LXR-a (Liver X Receptor alpha) ligand binding domain is a 55.9 kDa recombinant human protein (amino acids 207-447) expressed as a GST fusion protein in baculovirus-infected insect cells. LXR alpha was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.

Protein Form: Recombinant, Ligand Binding Domain

Target Information

NR1H3 (oxysterols receptor LXR-alpha) is a nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Its interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides. NR1H3 plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8. It also interplays functionally with RORA for the regulation of genes involves in liver metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.