Thermo Fisher Scientific CD205 Monoclonal Antibody (205yekta), Alexa Fluor 488, eBioscience
Alexa Fluor 488로 결합된 mouse CD205 단클론 항체(205yekta)로, Flow Cytometry에 최적화되어 있습니다. 항원 포획 및 제시 과정 연구에 적합하며, 0.25 µg/test로 사용 가능. 4°C 암소 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 205yekta |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_10734965 |
Additional Formats Available
- APC
- Biotin
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The 205yekta antibody reacts with mouse CD205 (DEC-205), a 205 kDa integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation that enhances antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells and also found in bone marrow Gr1+ cells, Langerhans cells, BMDCs, and thymic epithelial cells.
CD205 binds to PE-Cy5.5 antibody and streptavidin conjugates; these formats should not be used on CD205+ dendritic cells.
Applications Reported:
- Flow cytometric analysis
Applications Tested:
- Tested by flow cytometric analysis of mouse bone marrow cells
- Recommended usage ≤ 0.25 µg per test (100 µL final volume)
- Cell number range: 10⁵–10⁸ cells/test
- Optimize antibody titration for best performance
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD205 (LY25, Lymphocyte Antigen 75):
An integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation, enhancing antigen-specific T cell immunity. Highly expressed by CD8+ dendritic cells and variably expressed in bone marrow Gr1+ cells, Langerhans cells, BMDCs, and thymic epithelial cells. Acts as an endocytic receptor directing captured antigens to specialized antigen-processing regions and may reduce proliferation of B lymphocytes.
Associated diseases:
- Pneumonic plague
- Lipid pneumonia
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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