Thermo Fisher Scientific GOLPH3 Polyclonal Antibody

Applications

Immunoprecipitation (IP)

• Tested Dilution: 15–20 µL/mg of lysate

Product Specifications

Product Specific Information

Predicted reactivity by homology: Rat, Mouse.

Target Information

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin.
GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to an increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF.
Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA-treated cells compared to control cells, indicating that GOLPH3 can enhance signaling through TOR-associated complexes.
These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.