Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:10,000

Immunohistochemistry (IHC)

1:500-1:2,000

ELISA (ELISA)

1:500-1:2,000

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

HRP HRP HRP

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Goat/)
• [AP](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Goat/)
• [APC ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/APC/host/Goat/)
• [Cyanine3](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Cyanine3/host/Goat/)
• [Cyanine5 ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Cyanine5/host/Goat/)
• [DSB-X Biotin](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/DSB-X Biotin/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Goat/)
• [DyLight 755](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Goat/)
• [DyLight 800](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/PE/host/Goat/)
• [Texas Red-X](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/Texas Red-X/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rat IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_10561556

Target

IgG

Cross Adsorption

Against human IgG, human serum, mouse IgG, mouse serum and bovine serum

Antibody Form

Whole Antibody

Product Specific Information

Storage and reconstitution: The formulation of this antibody was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024, any lots produced after this date will not contain thimerosal. Lot numbers greater than 2855945 will not have thimerosal.

Store product at -20°C until opened. Reconstitute using 0.5 mL PBS, pH 7.2, to yield a 1 mg/mL stock solution. After reconstitution in buffer without a stabilizer like thimerosal, the product is stable for approximately three months at 4°C. For prolonged storage after reconstitution in buffers without a stabilizer, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C. The antibody will be stable for up to one year stored in this manner.

Alternatively, if the product is reconstituted in a buffer containing a preservative, for example, in PBS, pH 7.2, containing 0.02% thimerosal, the product will be stable for up to one year when stored at 4°C.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.