
Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합된 염소 유래 anti-Rat IgG(H+L) 2차항체로, WB, IHC, ELISA에 적합합니다. 교차흡착 처리로 비특이적 반응을 최소화하며, 동결건조 형태로 제공됩니다. PBS로 재구성 후 안정적이며, 높은 신호 증폭 효율을 제공합니다.
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Applications and Tested Dilutions
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_10561556 |
| Target | IgG |
| Cross Adsorption | Against human IgG, human serum, mouse IgG, mouse serum, and bovine serum |
| Antibody Form | Whole Antibody |
Product Specific Information
Storage and Reconstitution
- The formulation was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024.
- Lot numbers greater than 2855945 will not contain thimerosal.
- Store product at -20°C until opened.
- Reconstitute using 0.5 mL PBS (pH 7.2) to yield a 1 mg/mL stock solution.
- After reconstitution in buffer without a stabilizer, the product is stable for approximately 3 months at 4°C.
- For prolonged storage, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C (stable for up to 1 year).
- If reconstituted in buffer containing a preservative (e.g., PBS with 0.02% thimerosal), it is stable for up to 1 year at 4°C.
Target Information
Anti-Rat secondary antibodies are affinity-purified with high specificity for rat immunoglobulins.
They are useful for detection, sorting, or purification of rat targets.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody.
Typically, they are generated by immunizing the host with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation).
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
Available Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Cyanine3, Cyanine5, DSB-X Biotin
- DyLight 488, 550, 594, 650, 680, 755, 800
- FITC, PE, Texas Red-X, TRITC
- Custom conjugation available upon request
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