Thermo Fisher Scientific eIF3i Polyclonal Antibody
Human eIF3i 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot 및 Immunocytochemistry에 적합. Protein A로 정제된 고순도 항체이며, PBS/glycerol buffer에 보관. 다양한 종에서 예측 반응성을 보이며 연구용으로 사용 가능.
- 카탈로그번호
- PA527582
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 66–325 of Human EIF3 beta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS, pH 7, with 40% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2545058 |
Product Specific Information
- Recommended positive controls: A431, H1299, HeLa, HepG2
- Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (88%), Pig (98%), Rabbit (96%), Chicken (92%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC).
The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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