Thermo Fisher Scientific FEN1 Polyclonal Antibody
Rabbit polyclonal antibody against human FEN1 for WB and IHC applications. High specificity and affinity purified by antigen chromatography. Supplied in PBS with glycerol, suitable for research use in DNA replication and repair studies.
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human FEN1. Recombinant protein control fragment (Product #RP-89493). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2789421 |
Product Specific Information
Immunogen sequence:
CAALVKAGKV YAAATEDMDC LTFGSPVLMR HLTASEAKKL PIQEFHLSRI LQELGLNQEQ FVDLCILLGS DYCESIRGIG PKRAVDLIQK HKSIEEIVRR LDPNKYPVPE NWLHKEAHQL FLEPEVLDPE SVELKW
Target Information
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein functioning as an endonuclease that cleaves branched DNA structures containing single-stranded 5′ flaps or as an exonuclease on DNA nicks and gaps.
FEN-1 endonuclease and exonuclease activities are stimulated by association with proliferating cell nuclear antigen (PCNA) in the nucleus.
It plays important roles in DNA replication and repair and is required in cell-free DNA replication systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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