Thermo Fisher Scientific ERK1/ERK2 Polyclonal Antibody
ERK1/ERK2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, IP에 사용 가능. 다양한 종에 반응하며 세포 신호전달 연구에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 4 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | 1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rat, Yeast, Zebrafish |
| Published Species | Human, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a sequence in the C-terminus of rat p44 MAP Kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1185592 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric G protein-coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways.
When growth factors bind to receptor tyrosine kinase, Ras interacts with Raf (a serine/threonine protein kinase) and activates it. Activated Raf phosphorylates MEK1/2, which in turn phosphorylates tyrosine/threonine residues in ERK1/2.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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