Thermo Fisher Scientific Ki-67 Polyclonal Antibody
Ki-67 단백질을 인식하는 Rabbit Polyclonal 항체로, 세포 증식 마커 연구에 사용됩니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, Human, Mouse, Rat 시료에 반응합니다. Protein A로 정제되어 고순도이며, 4°C 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | – | View 1 publication |
| Immunohistochemistry (IHC) | – | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10–20 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 3 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from the human Ki-67 antigen |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 0.2% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_559806 |
Product Specific Information
- Recommended positive controls: Tonsil or Breast carcinoma.
- Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. The protein contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus. Ki-67 displays cell cycle-dependent localization: perinucleolar expression in G1, nuclear matrix expression in G2, and chromosomal expression during M phase.
As a proliferation marker, Ki-67 is absent in G0 cells but increases from G1 through mitosis. Ki-67 antibodies are valuable for assessing proliferative activity in neoplasms, showing strong correlation between low Ki-67 index and low-grade tumors. It is routinely used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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